期刊
ACS APPLIED MATERIALS & INTERFACES
卷 13, 期 17, 页码 19816-19824出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.1c04453
关键词
SARS-CoV-2; RNA-dependent RNA polymerase; MXene-Ti3C2; DNA walker; electrochemiluminescence; biosensor
资金
- National Natural Science Foundation of China [21705061]
- Jiangsu Provincial Key Medical Discipline (Laboratory) [ZDXKA2016017]
- Innovation Capacity Development Plan of Jiangsu Province [BM2018023]
A novel Au@Ti3C2@PEI-Ru(dcbpy)(3)(2+) nanocomposite-based ECL biosensor was designed for the sensitive detection of the SARS-CoV-2 RdRp gene. The sensor showed a wide detection range and a low limit of detection, indicating its great potential for clinical medical applications. The use of a DNA walker amplification strategy also showed promising results for other detection methods.
The detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial for preventing and controlling infectious diseases and disease treatment. In this work, a Au@Ti3C2@PEI-Ru(dcbpy)(3)(2+) nanocomposite-based electrochemiluminescence (ECL) biosensor was rationally designed, which realized sensitive detection of the RNA-dependent RNA polymerase (RdRp) gene of SARS-CoV-2. In addition, a DNA walker was also used to excise the hairpin DNAs under the action of Nb.BbvCI endonuclease. Furthermore, model DNA-Ag nanoclusters (model DNA-AgNCs) were used to quench the initial ECL signal. As a result, the ECL biosensor was used to sensitively detect the SARS-CoV-2 RdRp gene with a detection range of 1 fM to 100 pM and a limit of detection of 0.21 fM. It was indicated that the ECL biosensor had a great application potential for clinical medical detection. Furthermore, the DNA walker amplification also played a reliable candidate strategy for other detection methods.
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