期刊
ACS APPLIED MATERIALS & INTERFACES
卷 13, 期 17, 页码 19581-19592出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.0c23096
关键词
microfluidics; antibiotic susceptibility testing; microchambers; flourescence-based detection; resazurin
资金
- United States Department of Agriculture's National Institute of Food and Agriculture [2019-38420-28975]
- National Science Foundation [NNCI-2025233]
The EL-MVM2 platform quickly generates a wide range of gradient-based antibiotic concentrations with high precision, making it an effective, time-saving, and precise alternative to conventional antibiotic susceptibility testing platforms currently used in clinical diagnostics and point-of-care settings.
Fast determination of antimicrobial agents' effectiveness (susceptibility/resistance pattern) is an essential diagnostic step for treating bacterial infections and stopping worldwide outbreaks. Here, we report an egg-like multivolume microchamber-based microfluidic (EL-MVM2) platform, which is used to produce a wide range of gradient-based antibiotic concentrations quickly (similar to 10 min). The EL-MVM2 platform works based upon testing a bacterial suspension in multivolume microchambers (microchamber sizes that range from a volume of 12.56 to 153.86 nL). Antibiotic molecules from a stock solution diffuse into the microchambers of various volumes at the same loading rate, leading to different concentrations among the microchambers. Therefore, we can quickly and easily produce a robust antibiotic gradient-based concentration profile. The EL-MVM2 platform's diffusion (loading) pattern was investigated for different antibiotic drugs using both computational fluid dynamics simulations and experimental approaches. With an easy-to-follow protocol for sample loading and operation, the EL-MVM2 platform was also found to be of high precision with respect to predicting the susceptibility/resistance outcome (>97%; surpassing the FDA-approval criterion for technology-based antimicrobial susceptibility testing instruments). These features indicate that the EL-MVM2 is an effective, time-saving, and precise alternative to conventional antibiotic susceptibility testing platforms currently being used in clinical diagnostics and point-of-care settings.
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