期刊
ISCIENCE
卷 24, 期 2, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.isci.2021.102040
关键词
-
资金
- National Natural Science Foundation of China [31872286]
This study reveals a regulatory mechanism involving intragenic DNA methylation that controls ovarian and embryonic developmental processes in the silkworm Bombyx mori, with DNA methylation enhancing gene expression near the transcription start site of ovarian genes. Additionally, methyl-DNA-binding domain proteins MBD2/3 bind to intragenic methyl-CpG fragments, recruiting the acetyltransferase Tip60 to promote histone H3K27 acetylation and gene expression.
DNA methylation is an important epigenetic modification. However, the regulations and functions of insect intragenic DNA methylation remain unknown. Here, we demonstrate that a regulatory mechanism involving intragenic DNA methylation controls ovarian and embryonic developmental processes in Bombyx mori. In B. mori, DNA methylation is found near the transcription start site (TSS) of ovarian genes. By promoter activity analysis, we observed that 5' UTR methylation enhances gene expression. Moreover, methyl-DNA-binding domain protein 2/3 (MBD2/3) binds to the intragenic methyl-CpG fragment and recruits acetyltransferase Tip60 to promote histone H3K27 acetylation and gene expression. Additionally, genome-wide analyses showed that the peak of H3K27 acetylation appears near the TSS of methyl-modified genes, and DNA methylation is enriched in genes involved in protein synthesis in the B. mori ovary, with MBD2/3 knockdown resulting in decreased fecundity. These data uncover a mechanism of gene body methylation for regulating insect gene expression and reproduction.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据