Recombination machinery engineering for precise genome editing in methylotrophic yeast Ogataea polymorpha

Methanol biotransformation can expand biorefinery substrate spectrum other than biomass by using methylotrophic microbes. Ogataea (Hansenula) polymorpha, a representative methylotrophic yeast, attracts much attention due to its thermotolerance, but the low homologous recombination (HR) efficiency hinders its precise genetic manipulation during cell factory construction. Here, recombination machinery engineering (rME) is explored for enhancing HR activity together with establishing an efficient CRISPR-Cas9 system in O. polymorpha. Overexpression of HR-related proteins and down-regulation of non-homologous end joining (NHEJ) increased HR rates from 20%-30% to 60%-70%. With these recombination perturbation mutants, a competition between HR and NHEJ is observed. This HR up-regulated system has been applied for homologous integration of large fragments and in vivo assembly of multiple fragments, which enables the production of fatty alcohols in O. polymorpha. These findings will simplify genetic engineering in non-conventional yeasts and facilitate the adoption of O. polymorpha as an attractive cell factory for industrial application.

Automated summary

The study focuses on improving homologous recombination (HR) activity in O. polymorpha through recombination machinery engineering (rME) and establishing an efficient CRISPR-Cas9 system. By overexpressing HR-related proteins and down-regulating non-homologous end joining (NHEJ), HR rates were significantly increased. This enhanced HR system has allowed for successful homologous integration of large fragments and in vivo assembly of multiple fragments in order to produce fatty alcohols.