期刊
JOURNAL OF FUNGI
卷 7, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/jof7020131
关键词
analytical chemistry; Ascomycota; bioactivity screening chemotaxonomy; molecular phylogenetics; polyphasic taxonomy; two new species
资金
- University of Guilan, Iran
This study focused on the investigation of the Hypoxylon fuscum species complex through morphological studies and chemical analysis, resulting in the discovery of two unprecedented molecules. A new daldinin derivative 6 showed weak cytotoxicity towards mammalian cells and bactericidal activity. The new cytochalasin 4 exhibited comparable activity to known compounds in disrupting F-actin, but with irreversible effects.
Hypoxylon, a large, cosmopolitan genus of Ascomycota is in the focus of our current poly-thetic taxonomic studies, and served as an excellent source for bioactive secondary metabolites at the same time. The present work concerns a survey of the Hypoxylon fuscum species complex based on specimens from Iran and Europe by morphological studies and high performance liquid chromatography coupled to mass spectrometry and diode array detection (HPLC-MS-DAD). Apart from known chemotaxonomic markers like binaphthalene tetrol (BNT) and daldinin F, two unprece-dented molecules were detected and subsequently isolated to purity by semi preparative HPLC. Their structures were established by nuclear-magnetic resonance (NMR) spectroscopy as 3 '-malonyl-daldinin F (6) and pseudofuscochalasin A (4). The new daldinin derivative 6 showed weak cytotoxicity towards mammalian cells but bactericidal activity. The new cytochalasin 4 was compared to cytochalasin C in an actin disruption assay using fluorescence microscopy of human osteo-sarcoma U2OS cells, revealing comparable activity towards F-actin but being irreversible compared to cytochalasin C. Concurrently, a multilocus molecular phylogeny based on ribosomal and proteinogenic nucleotide sequences of Hypoxylon species resulted in a well-supported clade for H. fuscum and its allies. From a comparison of morphological, chemotaxonomic and phylogenetic evidence, we introduce the new species H. eurasiaticum and H. pseudofuscum.
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