期刊
BIOMEDICINES
卷 9, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/biomedicines9030278
关键词
mesenchymal stem cell; stem cell; niche; junctional adhesion molecule; tight junction; shedding
资金
- JSPS KAKENHI [18K15127, 20K21529]
- AMED [JP20lm0203002]
- Grants-in-Aid for Scientific Research [20K21529, 18K15127] Funding Source: KAKEN
Junctional adhesion molecules (JAMs) have been found to exhibit a novel mode of interaction and biological activity in adipose-derived stromal/stem cells, with JAM-C playing a significant role in cell adhesion and proliferation. The cleaved form of JAM-C, sJAM-C, can stimulate adhesion and maintenance of ADSCs in conjunction with JAM-B. These findings suggest a unique functional role of sJAM-C in ADSCs, contributing to not only cell-cell adhesion, but also cell-matrix adhesion.
Junctional adhesion molecules (JAMs) are expressed in diverse types of stem and progenitor cells, but their physiological significance has yet to be established. Here, we report that JAMs exhibit a novel mode of interaction and biological activity in adipose-derived stromal/stem cells (ADSCs). Among the JAM family members, JAM-B and JAM-C were concentrated along the cell membranes of mouse ADSCs. JAM-C but not JAM-B was broadly distributed in the interstitial spaces of mouse adipose tissue. Interestingly, the JAM-C ectodomain was cleaved and secreted as a soluble form (sJAM-C) in vitro and in vivo, leading to deposition in the fat interstitial tissue. When ADSCs were grown in culture plates coated with sJAM-C, cell adhesion, cell proliferation and the expression of five mesenchymal stem cell markers, Cd44, Cd105, Cd140a, Cd166 and Sca-1, were significantly elevated. Moreover, immunoprecipitation assay showed that sJAM-C formed a complex with JAM-B. Using CRISPR/Cas9-based genome editing, we also demonstrated that sJAM-C was coupled with JAM-B to stimulate ADSC adhesion and maintenance. Together, these findings provide insight into the unique function of sJAM-C in ADSCs. We propose that JAMs contribute not only to cell-cell adhesion, but also to cell-matrix adhesion, by excising their ectodomain and functioning as a niche-like microenvironment for stem and progenitor cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据