期刊
BIOMEDICINES
卷 9, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/biomedicines9020203
关键词
bone marrow mesenchymal stem cells; migration; proliferation; platelet-derived growth factor; stromal cell-derived factor-1 alpha
资金
- Department of Veterans Affairs, United States [I01RX001991-01A2]
This study demonstrated the high viability of bone marrow mesenchymal stem cells (bMSCs) in Gelatin-hydroxyphenyl propionic acid (Gtn-HPA) covalent cross-linking gelation. The incorporation of platelet-derived growth factor (PDGF)-BB directly into the gel or released from polyelectrolyte complex nanoparticles (PCNs) stimulated bMSC migration and proliferation effectively. Stromal cell-derived factor (SDF)-1 alpha enhanced migration and proliferation when released from PCNs, highlighting the potential of using Gtn-HPA/PDGF-BB as an injectable therapeutic agent for musculoskeletal tissue defects.
Bone marrow mesenchymal stem cells (bMSCs) are responsible in the repair of injured tissue through differentiation into multiple cell types and secretion of paracrine factors, and thus have a broad application profile in tissue engineering/regenerative medicine, especially for the musculoskeletal system. The lesion due to injury or disease may be a closed irregular-shaped cavity deep within tissue necessitating an injectable biomaterial permissive of host (endogenous) cell migration, proliferation and differentiation. Gelatin-hydroxyphenyl propionic acid (Gtn-HPA) is a natural biopolymer hydrogel which is covalently cross-linked by horseradish peroxidase (HRP) and hydrogen peroxide (H2O2) in situ and can be delivered to the lesion by needle injection. Growth factors and cytokines can be directly incorporated into the gel or into nano- and micro-particles, which can be employed for sustained release of biomolecules while maintaining their bioactivity. In this study, we selected polyelectrolyte complex nanoparticles (PCNs) prepared with dextran sulfate and chitosan as the carrier for platelet-derived growth factor (PDGF)-BB and stromal cellderived factor (SDF)-1 alpha, which have been tested effectively in recruiting stem cells. Our in vitro results showed a high degree of viability of bMSCs through the process of Gtn-HPA covalent cross-linking gelation. The Gtn-HPA matrix was highly permissive of bMSC migration, proliferation, and differentiation. PDGF-BB (20 ng/mL) directly incorporated into the gel and, alternatively, released from PCNs stimulated bMSC migration and proliferation. There were only small differences in the results for the direct incorporation of PDGF into the gel compared with its release from PCNs, and for increased doses of the growth factor (200 ng/mL and 2 mu g/mL). In contrast, SDF-1 alpha elicited an increase in migration and proliferation only when released from PCNs; its effect on migration was notably less than PDGF-BB. The in vitro results demonstrate that PDGF-BB substantially increases migration of bMSCs into Gtn-HPA and their proliferation in the gel, and that these benefits can be derived from incorporation of a relatively low dose of the growth factor directly into the gel. These findings commend the use of Gtn-HPA/PDGF-BB as an injectable therapeutic agent to treat defects in musculoskeletal tissues.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据