期刊
BIOACTIVE MATERIALS
卷 6, 期 2, 页码 333-345出版社
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2020.08.015
关键词
Multi-walled carbon nanotubes (MCNTs); Bone repair material; Protein adsorption; Osteogenic differentiation; Bone formation
资金
- National Natural Science Foundation of China [31771042]
- Fok Ying Tung Education Foundation [141039]
- State Key Laboratory of New Ceramic and Fine Processing Tsinghua University
- Key Laboratory of Advanced Materials of Ministry of Education [2020AML10]
- International Joint Research Center of Aerospace Biotechnology and Medical Engineering, Ministry of Science and Technology of China
- 111 Project [B13003]
The study found that multi-walled carbon nanotubes (MCNTs) were more effective in activating specific signaling pathways than nano-hydroxyapatite (nHA) and could better promote osteogenic differentiation of human adipose-derived mesenchymal stem cells (HASCs). In in vivo experiments, MCNTs were also found to induce ectopic bone formation better than nHA.
It has been well recognized that the development and use of artificial materials with high osteogenic ability is one of the most promising means to replace bone grafting that has exhibited various negative effects. The biomimetic features and unique physiochemical properties of nanomaterials play important roles in stimulating cellular functions and guiding tissue regeneration. But efficacy degree of some nanomaterials to promote specific tissue formation is still not clear. We hereby comparatively studied the osteogenic ability of our treated multi-walled carbon nanotubes (MCNTs) and the main inorganic mineral component of natural bone, nano-hydroxyapatite (nHA) in the same system, and tried to tell the related mechanism. In vitro culture of human adipose-derived mesenchymal stem cells (HASCs) on the MCNTs and nHA demonstrated that although there was no significant difference in the cell adhesion amount between on the MCNTs and nHA, the cell attachment strength and proliferation on the MCNTs were better. Most importantly, the MCNTs could induce osteogenic differentiation of the HASCs better than the nHA, the possible mechanism of which was found to be that the MCNTs could activate Notch involved signaling pathways by concentrating more proteins, including specific bone-inducing ones. Moreover, the MCNTs could induce ectopic bone formation in vivo while the nHA could not, which might be because MCNTs could stimulate inducible cells in tissues to form inductive bone better than nHA by concentrating more proteins including specific bone-inducing ones secreted from M2 macrophages. Therefore, MCNTs might be more effective materials for accelerating bone formation even than nHA.
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