Transmission Structured Illumination Microscopy for Quantitative Phase and Scattering Imaging

In this paper, we demonstrate a digital micromirror device (DMD) based optical microscopic apparatus for quantitative differential phase contrast (qDIC) imaging, coherent structured illumination microscopy (SIM), and dual-modality (scattering/fluorescent) imaging. For both the qDIC imaging and the coherent SIM, two sets of fringe patterns with orthogonal orientations and five phase-shifts for each orientation, are generated by a DMD and projected on a sample. A CCD camera records the generated images in a defocusing manner for qDIC and an in-focus manner for coherent SIM. Both quantitative phase images and super-resolved scattering/fluorescence images can be reconstructed from the recorded intensity images. Moreover, fluorescent imaging modality is integrated, providing specific biochemical structures of the sample once using fluorescent labeling.

Automated summary

This paper demonstrates a DMD-based optical microscopic apparatus for qDIC imaging, coherent SIM, and dual-modality imaging. By projecting two sets of fringe patterns with orthogonal orientations on the sample, quantitative phase images and super-resolved scattering/fluorescence images can be reconstructed. Additionally, fluorescent imaging modality is integrated to provide specific biochemical structures of the sample.