期刊
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
卷 23, 期 10, 页码 9843-9851出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-016-6095-5
关键词
In vitro bioassay; Toxicmetals; Thyroid receptor; Thyroid-disrupting activity
资金
- National Natural Science Foundation of China [41001351]
- Fundamental Research Funds for the Central Universities [2012LYB35]
- Major Science and Technology Program for Water Pollution Control and Treatment [2014ZX07201-010]
In the present study, a two-hybrid yeast bioassay and a T-screen were used to screen for the thyroid receptor (TR)-disrupting activity of select metallic compounds (CdCl2, ZnCl2, HgCl2, CuSO4, MnSO4, and MgSO4). The results reveal that none of the tested metallic compounds showed TR-agonistic activity, whereas ZnCl2, HgCl2, and CdCl2 demonstrated TR antagonism. For the yeast assay, the dose-response relationship of these metallic compounds was established, and the concentrations producing 20 % of the maximum effect of ZnCl2, HgCl2, and CdCl2 were 9.1 x 10(-5), 3.2 x 10(-6), and 1.2 x 10(-6) mol/L, respectively. The T-screen also supported the finding that ZnCl2, HgCl2, and CdCl2 decreased the cell proliferation at concentrations ranging from 10(-6) to 10(-4) mol/L. Furthermore, the thyroid-disrupting activity of metallic compounds in environmental water samples collected from the Guanting Reservoir, Beijing, China was evaluated. Solid-phase extraction was used to separate the organic extracts, and a modified two-hybrid yeast bioassay revealed that the metallic compounds in the water samples could affect thyroid hormone-induced signaling by decreasing the binding of the thyroid hormone. The addition of ethylenediaminetetraacetic acid (30 mg/L) could eliminate the effects. Thus, the cause(s) of the thyroid toxicity in the water samples appeared to be partly related to the metallic compounds.
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