期刊
FOODS
卷 10, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/foods10030544
关键词
antibiotics; metabolites; non-targeted analysis; Compound Discoverer; milk; screening test; HPLC-HRMS; food safety
资金
- Piedmont Region as part of the Bovilat 4.0 project entitled Monitoring of the quality of bovine milk produced in the regional territory
- University of Milan through the APC initiative
This study aimed to verify the presence of antibiotic residues in milk after therapeutic treatments, showing that 29% of samples had antimicrobial drugs and their metabolites, with 9% of samples also revealing undisclosed treatments. Furthermore, two new enrofloxacin metabolites were proposed, highlighting the potential risk of metabolites to consumers and the processing of milk in the cheese industry.
Antibiotic residues in milk are a serious health and technological problem in dairy processes. This study aims to verify the absence of administered antimicrobials after therapeutic treatments, taking into consideration the withdrawal period, and to evaluate the reliability of screening tests under field conditions after confirmatory HPLC-HRMS (High Performance Liquid Chromatography-High-Resolution Mass Spectrometry) Orbitrap analysis. Moreover, the presence of expected or non-targeted metabolites was investigated using the new Compound Discoverer approach. The presence of antimicrobial drugs was shown in 29% of the samples, and also sometimes their metabolites (for enrofloxacin and lincomycin), despite the fact that samples were collected at the seventh milking. Moreover, in 9% of the samples, undeclared treatments were revealed due to the presence of both parent drugs and metabolites. Lastly, the putative identification of two new enrofloxacin metabolites, ENRO-N-methylacetamide and ENRO-ornithine, was proposed. In the light of this evidence, it must be borne in mind that metabolites, some of which are pharmacologically active, may also pose a risk to consumers and for the entire processing of milk in the cheese industries.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据