期刊
FOODS
卷 10, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/foods10020398
关键词
carao tree seeds; phenolics; PLE; HPLC-MS; bioactive compounds
资金
- Coimbra Group Scholarship Programme for Young Professors and Researchers from Latin American Universities
- Centro de Iniciativas de Cooperacion al desarrollo (CICODE)
- Research Group Ciencias de la Alimentacion y Nutricion (University of Granada, Spain) [AGR279]
- Universidad Nacional de Agricultura, Catacamas, Honduras
Several studies have shown the protective effects of bioactive compounds in food against chronic diseases, with a particular focus on phenolic compounds. This study aimed to determine the bioactive composition of Carao tree seeds and optimize the extraction process. Analysis revealed that Carao tree seeds could be a potential source of bioactive compounds.
Nowadays there are evidences from several studies which have revealed the protective effects of food against chronic diseases. These healthy properties have been related to bioactive compounds. Among bioactive substances, the scientific interest in phenolic compounds has stimulated multidisciplinary research on the composition of plant phenolic compounds. The aim of this work has been to determine the bioactive composition of Carao tree seeds (Cassia grandis) and to optimize the recovering of these compounds for developing functional ingredients. To achieve this goal, pressurized liquid extraction (PLE) has been applied to recover these phytochemicals. The optimization of this innovative extraction procedure was performed by a response surface methodology (RSM) based on a central composite design 2(3) model to address the bioactive compounds extraction. Phenolic compounds recovered by PLE were characterized using reversed-phase high-performance liquid chromatography coupled to electrospray ionization time-of-flight mass spectrometry (HPLC-ESI-TOF-MS). Analytical characterization allowed the identification and quantitation of phenolic compounds belonging to hydroxybenzoic acids and flavonoids (flavonols, flavanols, flavanones and proanthocyanidins). Phytochemical concentrations were used as response variable in order to get the best extraction conditions. These results pointed out that Carao tree seeds can be a potential source of bioactive compounds and PLE extracts could be used as functional ingredients.
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