期刊
REGENERATIVE BIOMATERIALS
卷 8, 期 2, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/rb/rbaa057
关键词
peptide hydrogel; cell-adhesive ligand; human amniotic mesenchymal stem cells; stemness maintenance; osteogenesis differentiation; integrin
资金
- National Natural Science Foundation of China [31360232]
- Natural Science Research project of Education Department of Guizhou Province [Qian Jiao He KY Zi [2015]418]
The study demonstrates that incorporating cell-adhesive ligands in 3D self-assembling peptide hydrogels is effective for maintaining stem cell survival, proliferation, and enhancing osteogenic differentiation. The composite hydrogels also upregulate the expression of pluripotent markers in stem cells, indicating their potential for modulating cell fate.
In vivo, stem cells reside in a three-dimensional (3D) extracellular microenvironment in which complicated biophysical and biochemical factors regulate their behaviors. Biomimicking of the stem cell-matrix interactions is an ideal approach for controlling the stem cell fate. This study investigates the effects of the incorporation of cell-adhesive ligands in 3D self-assembling peptide hydrogels to modulate stem cell survival, proliferation, maintenance of stemness, and osteogenic differentiation. The results show that the composite hydrogels were non-cytotoxic and effective for maintaining human amniotic mesenchymal stem cell (hAMSC) survival, proliferation and phenotypic characterization. The expression levels of pluripotent markers were also upregulated in the composite hydrogels. Under inductive media conditions, mineral deposition and mRNA expression levels of osteogenic genes of hAMSCs were enhanced. The increasing expression of integrin alpha- and beta-subunits for hAMSCs indicates that the ligand-integrin interactions may modulate the cell fate for hAMSCs in composite hydrogels.
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