期刊
MICROORGANISMS
卷 9, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms9020446
关键词
broiler; Salmonella Kentucky; Salmonella Typhimurium; Salmonella Enteritidis; colonization resistance; poultry; immune response; cytokine
类别
The study revealed competitive exclusion among Salmonella serotypes in neonatal broilers, potentially reducing colonization and impacting the host immune response. Changes in gut colonization and systemic translocation, as well as significant alterations in mRNA levels of cytokines, suggest a profound effect on local and systemic immune responses.
Salmonellosis is a zoonotic infection caused by Salmonella enterica serotypes contracted from contaminated products. We hypothesized that competitive exclusion between Salmonella serotypes in neonatal broilers would reduce colonization and affect the host immune response. Day of hatch broilers were randomly allocated to one of six treatment groups: (1) control, which received saline, (2) Salmonella Kentucky (SK) only on day 1 (D1), (3) Salmonella Typhimurium (ST) or Salmonella Enteritidis (SE) only on D1, (4) SK on D1 then ST or SE on day 2 (D2), (5) ST or SE on D1 then SK on D2, and (6) SK and ST or SE concurrently on D1. Salmonella gut colonization and incidence were measured from cecal contents. Livers and spleens were combined and macerated to determine systemic translocation. Relative mRNA levels of interleukin-1 beta (IL-1 beta), IL-6, IL-10, IL-18, and gamma interferon (IFN-gamma) were measured in cecal tonsils and liver to investigate local and systemic immune responses. When a serotype was administered first, it was able to significantly reduce colonization of the following serotype. Significant changes were found in mRNA expression of cytokines. These results suggest competitive exclusion by Salmonella enterica serotypes affect local and systemic immune responses.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据