期刊
MICROORGANISMS
卷 9, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms9020418
关键词
membrane association; topology; cilevirus; movement protein; p29 capsid protein
类别
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2014/0845-9, 2017/50222-0, 2015/10249-1, 2017/19898-8]
- Spanish Agencia Estatal de Investigacion (AEI) [BIO2017-88321-R]
- Fondo Europeo de Desarrollo Regional (FEDER)
- Generalitat Valenciana [GV2015/010]
The study revealed the association of citrus leprosis virus C2 movement protein and capsid protein with biological membranes, with both proteins exposing their N- and C-termini to the cell cytoplasmic compartment. These findings have important implications for the intracellular movement of the virus.
Although citrus leprosis disease has been known for more than a hundred years, one of its causal agents, citrus leprosis virus C2 (CiLV-C2), is poorly characterized. This study described the association of CiLV-C2 movement protein (MP) and capsid protein (p29) with biological membranes. Our findings obtained by computer predictions, chemical treatments after membrane fractionation, and biomolecular fluorescence complementation assays revealed that p29 is peripherally associated, while the MP is integrally bound to the cell membranes. Topological analyses revealed that both the p29 and MP expose their N- and C-termini to the cell cytoplasmic compartment. The implications of these results in the intracellular movement of the virus were discussed.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据