期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2021.632230
关键词
Streptomyces; natural products; biosynthetic gene cluster; heterologous expression; in situ activation; synthetic biology
资金
- Key-Area Research and Development Program of Guangdong Province [2020B0303070002]
- National Natural Science Foundation of China [32071426]
- National Key R&D Program of China [2018YFA0903300]
- Natural Science Foundation of Tianjin Province [19JCYBJC24200]
This review discusses the commonly used strategies for activating silent BGCs in Streptomyces, focusing on approaches applied in heterologous and native hosts to accelerate the discovery of natural products.
Natural products (NPs) are critical sources of drug molecules for decades. About two-thirds of natural antibiotics are produced by Streptomyces. Streptomyces have a large number of secondary metabolite biosynthetic gene clusters (SM-BGCs) that may encode NPs. However, most of these BGCs are silent under standard laboratory conditions. Hence, activation of these silent BGCs is essential to current natural products discovery research. In this review, we described the commonly used strategies for silent BGC activation in Streptomyces from two aspects. One focused on the strategies applied in heterologous host, including methods to clone and reconstruct BGCs along with advances in chassis engineering; the other focused on methods applied in native host which includes engineering of promoters, regulatory factors, and ribosomes. With the metabolic network being elucidated more comprehensively and methods optimized more high-thoroughly, the discovery of NPs will be greatly accelerated.
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