期刊
RESEARCH
卷 2021, 期 -, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.34133/2021/2813643
关键词
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资金
- Special Research Funding on COVID19 from Sichuan Province of China [2020YFS0004]
- Science and Technology Department, Ministry of Education of the People's Republic of China
- NSFC [32071407, 62003023]
- 111 Project [B13003]
- Innovation Research Plan from the Shanghai Municipal Education Commission [ZXWF082101/056]
- Exploratory Project of Natural Science Foundation of Shanghai [19ZR1476000]
- Beijing Advanced Innovation Center for Biomedical Engineering at Beihang University
A platform integrating a portable detector and a novel microwell array biochip was developed for rapid detection of SARS-CoV-2, showing high sensitivity and specificity in clinical trials. The platform has the potential for rapid and user-friendly diagnosis of COVID-19 among other infectious pathogens.
Sensitive detection of SARS-CoV-2 is of great importance for inhibiting the current pandemic of COVID-19. Here, we report a simple yet efficient platform integrating a portable and low-cost custom-made detector and a novel microwell array biochip for rapid and accurate detection of SARS-CoV-2. The instrument exhibits expedited amplification speed that enables colorimetric read-out within 25 minutes. A polymeric chip with a laser-engraved microwell array was developed to process the reaction between the primers and the respiratory swab RNA extracts, based on reverse transcriptase loop-mediated isothermal amplification (RT-LAMP). To achieve clinically acceptable performance, we synthesized a group of six primers to identify the conserved regions of the ORF1ab gene of SARS-CoV-2. Clinical trials were conducted with 87 PCR-positive and 43 PCR-negative patient samples. The platform demonstrated both high sensitivity (95.40%) and high specificity (95.35%), showing potentials for rapid and user-friendly diagnosis of COVID-19 among many other infectious pathogens.
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