期刊
ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 50, 期 23, 页码 12602-12611出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.6b02138
关键词
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资金
- Fundamental Research Funds for the Central Universities [KYZ201518]
- China Postdoctoral Science Foundation [2015M580440]
- Natural Science Foundation of China [21210008]
- Innovative Research Team Development Plan of the Ministry of Education of China [IRT1256]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- 111 project [B12009]
- UK Biotechnology and Biological Sciences Research Council
- Biotechnology and Biological Sciences Research Council [BB/D002990/1, BBS/E/C/00004981, BBS/E/C/00005196] Funding Source: researchfish
- BBSRC [BB/D002990/1, BBS/E/C/00004960, BBS/E/C/00005196, BBS/E/C/00004981] Funding Source: UKRI
Land applications of municipal sewage sludge may pose a risk of introducing antibiotic resistance genes (ARGs) from urban environments into agricultural systems. However, how the sewage sludge recycling and application method influence soil resistome and mobile genetic elements (MGEs) remains unclear. In the present study, high throughput quantitative PCR was conducted on the resistome of soils from a field experiment with past (between 1994 and 1997) and annual (since 1994) applications of five different sewage sludges. Total inputs of organic carbon were similar between the two modes of sludge applications. Intrinsic soil resistome, defined as the ARGs shared by the soils in the control and sludge-amended plots, consisted of genes conferring resistance to multidrug, beta-lactam, Macrolide-Lincosamide-Streptogramin B (MLSB), tetracycline, vancomycin, and aminoglycoside, with multidrug resistance genes as the most abundant members. There was a strong correlation between the abundance of ARGs and MGE marker genes in soils. The composition and diversity of ARGs in the five sludges were substantially different from those in soils. Considerable proportions of ARGs and MGE marker genes in the sludges attenuated following the application, especially aminoglycoside and tetracycline resistance genes. Annual applications posed a more significant impact on the soil resistome, through both continued introduction and stimulation of the soil intrinsic ARGs. In addition, direct introduction of sludge-specific ARGs into soil was observed especially from ARG-rich sludge. These results provide a better insight into the characteristics of ARG dissemination from urban environment to the agricultural system through sewage sludge applications.
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