期刊
NPJ MICROGRAVITY
卷 7, 期 1, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41526-021-00131-1
关键词
-
资金
- First Class Fund of the 63rd Postdoctoral Foundation of China [2018M633683]
The combination of microgravity and radiation exacerbates the effects on MC3T3-E1 cells and rat femurs, leading to decreased cell activity, increased apoptosis rates, bone loss, and altered gene and protein expressions.
Microgravity is well-known to induce Osteopenia. However, the combined effects of microgravity and radiation that commonly exist in space have not been broadly elucidated. This research investigates the combined effects on MC3T3-E1 cells and rat femurs. In MC3T3-E1 cells, simulated microgravity and X-ray radiation, alone or combination, show decreased cell activity, increased apoptosis rates by flow cytometric analysis, and decreased Runx2 and increased Caspase-3 mRNA and protein expressions. In rat femurs, simulated microgravity and X-ray radiation, alone or combination, show increased bone loss by micro-CT test and Masson staining, decreased serum BALP levels and Runx2 mRNA expressions, and increased serum CTX-1 levels and Caspase-3 mRNA expressions. The strongest effect is observed in the combined group in MC3T3-E1 cells and rat femurs. These findings suggest that the combination of microgravity and radiation exacerbates the effects of either treatment alone on MC3T3-E1 cells and rat femurs.
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