期刊
FRONTIERS IN ONCOLOGY
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fonc.2021.620907
关键词
multi-drug resistance; heat shock protein 90; ovarian cancer; β -catenin; chemotherapy
类别
资金
- National Natural Science Foundation of China [81872451, 81301919, 31701212]
- Chengdu Science and Technology Bureau [2018-YF05-00672-SN]
- Application and Basic Project of Science and Technology Department of Sichuan Province [2019YJ0370]
- Major project of Education Department of Sichuan Province [17ZA0106]
- Natural Science Foundation of Chengdu Medical College [CYTD18-04]
- State Undergraduate Innovative Experiment Program [S202013705011]
The study revealed that overexpression of Hsp90 in multi-drug resistant ovarian cancer cells can be reduced to increase sensitivity to paclitaxel and cisplatin, decrease drug-induced apoptosis, and regulate the expressions of multidrug resistance proteins. Further, Hsp90 affects beta-catenin accumulation and transcriptional activity, contributing to multi-drug resistance through AKT/GSK3 beta signaling.
Ovarian cancer is the most lethal gynaecologic tumor, with which multi-drug resistance as the major therapeutic hindrance. Heat shock protein 90 (Hsp90) has been involved in cancer malignant behaviors. However, its role and mechanism in multi-drug resistance of ovarian cancer remains poorly understood. Our results demonstrated that Hsp90 was overexpressed in multi-drug resistant ovarian cancer cells. Hsp90 downregulation by shHsp90 or inhibitor BIIB021 increased the sensitivity of multi-drug resistant ovarian cancer cells to paclitaxel and cisplatin, and augmented the drugs-induced apoptosis. Hsp90 positively regulated the expressions of multi-drug resistance protein 1 (P-gp/MDR1), breast cancer resistance protein (BCRP), Survivin and Bcl-2 expressions closely associated with multi-drug resistance. Moreover, overexpression of Hsp90 promoted beta-catenin accumulation, while Hsp90 downregulation decreased the accumulation, nuclear translocation and transcriptional activity of beta-catenin. We also identified that beta-catenin was responsible for Hsp90-mediated expressions of P-gp, BCRP, Survivin, and Bcl-2. Furthermore, Hsp90 enhanced the AKT/GSK3 beta signaling, and AKT signaling played a critical role in Hsp90-induced accumulation and transcriptional activity of beta-catenin, as well as multi-drug resistance to paclitaxel and cisplatin. In conclusion, Hsp90 enhanced the AKT/GSK3 beta/beta-catenin signaling to induce multi-drug resistance of ovarian cancer. Suppressing Hsp90 chemosensitized multi-drug resistant ovarian cancer cells via impairing the AKT/GSK3 beta/beta-catenin signaling, providing a promising therapeutic strategy for a successful treatment of ovarian cancer.
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