期刊
CELLS
卷 10, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/cells10020423
关键词
miR-27b-3p; MSTN; myoblast; proliferation; differentiation
类别
资金
- Natural Science Foundation of Jiangsu Province [BK20181453]
- Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture [poultrylab2018-10]
- Special Funds Project for Transforming Scientific and Technological Achievements in Jiangsu Province [BA2018099]
- China Agriculture Research System [CARS-41]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
The study revealed that miR-27b-3p promotes the proliferation of CPMs by targeting MSTN and inhibits their differentiation. Overexpression of MSTN inhibits the proliferation and differentiation of CPMs, while interference with MSTN expression has the opposite effect.
microRNAs play an important role in the growth and development of chicken embryos, including the regulation of skeletal muscle genesis, myoblast proliferation, differentiation, and apoptosis. Our previous RNA-seq studies showed that microRNA-27b-3p (miR-27b-3p) might play an important role in regulating the proliferation and differentiation of chicken primary myoblasts (CPMs). However, the mechanism of miR-27b-3p regulating the proliferation and differentiation of CPMs is still unclear. In this study, the results showed that miR-27b-3p significantly promoted the proliferation of CPMs and inhibited the differentiation of CPMs. Then, myostatin (MSTN) was confirmed to be the target gene of miR-27b-3p by double luciferase reporter assay, RT-qPCR, and Western blot. By overexpressing and interfering with MSTN expression in CPMs, the results showed that overexpression of MSTN significantly inhibited the proliferation and differentiation of CPMs. In contrast, interference of MSTN expression had the opposite effect. This study showed that miR-27b-3p could promote the proliferation of CPMs by targeting MSTN. Interestingly, both miR-27b-3p and MSTN can inhibit the differentiation of CPMs. These results provide a theoretical basis for further understanding the function of miR-27b-3p in chicken and revealing its regulation mechanism on chicken muscle growth.
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