期刊
CELLS
卷 10, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/cells10020322
关键词
chloroplast translation; regulation; mRNA secondary structure; RNA structure probing; high light; gene expression; plastid; Arabidopsis thaliana
类别
资金
- Polish National Science Centre (Narodowe Centrum Nauki) [SONATA12, UMO-2016/23/D/NZ3/02491, MAESTRO6 2014/14/A/NZ1/00218]
- H2020 European Research Council (ERC) [StG2017-757411]
- H2020 Marie SklodowskaCurie Actions [MSCA-IF 703085]
- Novo Nordisk Foundation (Novo Nordisk Fonden) [NNF15OC0014202]
- Copenhagen Plant Science Centre Young Investigator Starting Grant
- German Science Foundation (Deutsche Forschungsgemeinschaft) [TR175]
- Independent Research Fund Denmark (Danmarks Frie Forskningsfond) [7014-00322B]
- VILLUM Foundation (Villum Fonden) [13363]
The study revealed that mRNA secondary structure influences translation in plastids, with increased accessibility of the translation initiation region of psbA mRNA after exposure to high light. The presence of a putative regulatory protein in the 5 ' UTR of psbA was identified, which may cause structural opening of the translation initiation region, facilitating ribosome access. This suggests that changes in mRNA secondary structure could represent a general mechanism for translational regulation of psbA and other plastid genes.
mRNA secondary structure influences translation. Proteins that modulate the mRNA secondary structure around the translation initiation region may regulate translation in plastids. To test this hypothesis, we exposed Arabidopsis thaliana to high light, which induces translation of psbA mRNA encoding the D1 subunit of photosystem II. We assayed translation by ribosome profiling and applied two complementary methods to analyze in vivo RNA secondary structure: DMS-MaPseq and SHAPE-seq. We detected increased accessibility of the translation initiation region of psbA after high light treatment, likely contributing to the observed increase in translation by facilitating translation initiation. Furthermore, we identified the footprint of a putative regulatory protein in the 5 ' UTR of psbA at a position where occlusion of the nucleotide sequence would cause the structure of the translation initiation region to open up, thereby facilitating ribosome access. Moreover, we show that other plastid genes with weak Shine-Dalgarno sequences (SD) are likely to exhibit psbA-like regulation, while those with strong SDs do not. This supports the idea that changes in mRNA secondary structure might represent a general mechanism for translational regulation of psbA and other plastid genes.
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