期刊
GENOMICS PROTEOMICS & BIOINFORMATICS
卷 19, 期 5, 页码 772-786出版社
ELSEVIER
DOI: 10.1016/j.gpb.2021.02.002
关键词
Expression pattern; Unknown protein; Pig; Proteome; Subcellular location
资金
- National Natural Science Foundations of China [31661143013]
- Jinxinnong Animal Science Development Foundation
This study identified a large number of unknown protein isoforms from normal pig tissues and systematically characterized them, enhancing the study of the pig proteome. Through high-throughput RNA sequencing, the functional annotations of these newly identified protein isoforms were analyzed, providing a basis for further improving genome annotation. Finally, a significant number of orthologous genes for unknown protein isoforms across multiple species were mined, referencing various pathways and providing valuable resources for pig genomics and biology research.
A lack of the complete pig proteome has left a gap in our knowledge of the pig genome and has restricted the feasibility of using pigs as a biomedical model. In this study, we developed a tissue-based proteome map using 34 major normal pig tissues. A total of 5841 unknown protein isoforms were identified and systematically characterized, including 2225 novel protein isoforms, 669 protein isoforms from 460 genes symbolized beginning with LOC, and 2947 protein isoforms without clear NCBI annotation in the current pig reference genome. These newly identified protein isoforms were functionally annotated through profiling the pig transcriptome with high-throughput RNA sequencing of the same pig tissues, further improving the genome annotation of the corresponding protein-coding genes. Combining the well-annotated genes that have parallel expression pattern and subcellular witness, we predicted the tissue-related subcellular locations and potential functions for these unknown proteins. Finally, we mined 3081 orthologous genes for 52.7% of unknown protein isoforms across multiple species, referring to 68 KEGG pathways as well as 23 disease signaling pathways. These findings provide valuable insights and a rich resource for enhancing studies of pig genomics and biology, as well as biomedical model application to human medicine.
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