期刊
SCIENCE ADVANCES
卷 7, 期 9, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abe7999
关键词
-
资金
- European Research Council T-Rex [682436]
- National Science Foundation Flanders (FWO) [G009415N, 3G017919]
- Czech Science Foundation [GA19-21758S]
- China Scholarship Council [201508440249]
- Ghent University BOF CSC [ST01511051]
- European Research Council (ERC) [682436] Funding Source: European Research Council (ERC)
This study reveals the molecular architecture of plant TSET complex and identifies the crucial roles of specific subunits in complex assembly and membrane interaction. The structural differences between the hexameric TSET in Dictyostelium and the octameric TPC in plants are highlighted, advancing the understanding of the endocytosis process.
Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi and animals, have retained the evolutionary ancient TSET complex as an endocytic regulator. Unlike other coatomer complexes, structural insight into TSET is lacking. Here, we reveal the molecular architecture of plant TSET [TPLATE complex (TPC)] using an integrative structural approach. We identify crucial roles for specific TSET subunits in complex assembly and membrane interaction. Our data therefore generate fresh insight into the differences between the hexameric TSET in Dictyostelium and the octameric TPC in plants. Structural elucidation of this ancient adaptor complex represents the missing piece in the coatomer puzzle and vastly advances our functional as well as evolutionary insight into the process of endocytosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据