期刊
HORTICULTURE RESEARCH
卷 8, 期 1, 页码 -出版社
NANJING AGRICULTURAL UNIV
DOI: 10.1038/s41438-021-00476-4
关键词
-
资金
- National Natural Science Foundation of China [31601743]
- Young Talent Development Program of Hubei Academy of Agricultural Sciences [Q2018027]
- Major Program for Technical Innovation of Hubei Province [2018ABA071]
- National Science Foundation [IOS 1444987]
- USDA grant (NIFA) [11889048]
The cultivated strawberry genome was reannotated based on PacBio and Illumina RNA libraries, resulting in an improved annotation v1.0.a2 with a high percentage of complete gene models. A strawberry genome database (SGD) was created for gene homolog searching and annotation downloading, facilitating genomic analyses and gene functional studies in strawberries and related species.
Cultivated strawberry (Fragaria x ananassa) is an important fruit crop species whose fruits are enjoyed by many worldwide. An octoploid of hybrid origin, the complex genome of this species was recently sequenced, serving as a key reference genome for cultivated strawberry and related species of the Rosaceae family. The current annotation of the F. ananassa genome mainly relies on ab initio predictions and, to a lesser extent, transcriptome data. Here, we present the structure and functional reannotation of the F. ananassa genome based on one PacBio full-length RNA library and ninety-two Illumina RNA-Seq libraries. This improved annotation of the F. ananassa genome, v1.0.a2, comprises a total of 108,447 gene models, with 97.85% complete BUSCOs. The models of 19,174 genes were modified, 360 new genes were identified, and 11,044 genes were found to have alternatively spliced isoforms. Additionally, we constructed a strawberry genome database (SGD) for strawberry gene homolog searching and annotation downloading. Finally, the transcriptome of the receptacles and achenes of F. ananassa at four developmental stages were reanalyzed and qualified, and the expression profiles of all the genes in this annotation are also provided. Together, this study provides an updated annotation of the F. ananassa genome, which will facilitate genomic analyses across the Rosaceae family and gene functional studies in cultivated strawberry.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据