期刊
HORTICULTURE RESEARCH
卷 8, 期 1, 页码 -出版社
NANJING AGRICULTURAL UNIV
DOI: 10.1038/s41438-020-00457-z
关键词
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资金
- National Key R&D Program of China [2018YFD1000200]
- National Natural Science Foundation of China [31772288, 31972378]
- Science and Technology Program of Yunnan Province [2019ZG002-1-03]
This study revealed that MdBT2 undergoes posttranslational modifications in response to nitrate deficiency, and interacts with MdGRF11, a 14-3-3 protein, which negatively regulates the stability of MdBT2 protein via a 26S proteasome-dependent pathway. This leads to increased abundance of MdMYB1 proteins and activation of anthocyanin biosynthesis-related genes. The results demonstrate the critical role of 14-3-3 proteins in the regulation of nitrate deficiency-induced anthocyanin accumulation.
Nitrogen is an important factor that affects plant anthocyanin accumulation. In apple, the nitrate-responsive BTB/TAZ protein MdBT2 negatively regulates anthocyanin biosynthesis. In this study, we found that MdBT2 undergoes posttranslational modifications in response to nitrate deficiency. Yeast two-hybrid, protein pull-down, and bimolecular fluorescence complementation (BiFC) assays showed that MdBT2 interacts with MdGRF11, a 14-3-3 protein; 14-3-3 proteins compose a family of highly conserved phosphopeptide-binding proteins involved in multiple physiological and biological processes. The interaction of MdGRF11 negatively regulated the stability of the MdBT2 protein via a 26S proteasome-dependent pathway, which increased the abundance of MdMYB1 proteins to activate the expression of anthocyanin biosynthesis-related genes. Taken together, the results demonstrate the critical role of 14-3-3 proteins in the regulation of nitrate deficiency-induced anthocyanin accumulation. Our results provide a novel avenue to elucidate the mechanism underlying the induction of anthocyanin biosynthesis in response to nitrate deficiency.
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