期刊
MATERIALS & DESIGN
卷 201, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.matdes.2021.109463
关键词
Enzyme-TiO(2 )composite; Modification of surface; Biocatalysis; Synergetic effect
资金
- National Natural Science Foundation of China [21873061]
This study immobilized HRP enzyme on TiO2 nanoparticles for catalyzing phenol degradation in wastewater, achieving high catalytic activity, stability, and reusability. A visual colorimetric method for phenol concentration detection was established using HRP-DNA-PTL@TiO2, showing potential for wastewater treatment applications with its synergistic enzymatic-photocatalytic effect.
In this work, TiO2 nano particle was used for two purposes: it played as a role of a solid carrier for immobilization of HRP, and meanwhile acted as a photo-catalyst itself. In this way, the loss of enzymatic activity during enzyme immobilization can be compensated by the coupling of TiO2 photo-catalysis. Then, the surface of TiO2 nanoparticles were modified by phase-transitioned lysozyme (FM) and connected with horseradish peroxidase (HRP) via a DNA helix (HRP-DNA-PTL@TiO2). The DNA double helix chain was utilized as a flexible spacer arm to keep the flexibility of the immobilized enzyme. The HRP-DNA-PTL@TiO2 showed a high recovered activity (90.52%) of the original activity of free HRP. This data is better than most reported activity recovery of immobilized enzyme. Moreover, HRP-DNA-FIFL@TiO2 can maintain high catalytic activity, stability and reusability when degrading phenol in wastewater. The phenol of 0.5 mmol.L-1 could be totally degraded within 10 min. Moreover, a visual colorimetric method for phenol concentration by HRP-DNA-PTL@TiO2 was established, and the phenol concentration of 0-0.2 mmol.L-1 can be visually detected with naked eyes. Furthermore, an enzymatic-photocatalytic synergetic effect of HRP-DNA-PTL@TiO2 is found and investigated. The efficiency of HRP-DNA-PTL@TiO2 ensures it a potential application in the wastewater treatment. (C) 2021 The Author(s). Published by Elsevier Ltd.
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