期刊
FRONTIERS IN IMMUNOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2021.603192
关键词
NOD1; A20; acute liver failure; LPS; D-GalN; apoptosis
类别
资金
- National Natural Science Foundation of China [81770570, 81801990]
- Program for Outstanding Young Talent of Chongqing Kuanren Hospital and Natural Science Foundation of Chongqing, China [cstc2019jcyj-msxmX0007]
- National Science and Technology Major Project of China [2017ZX10202203-007, 2017ZX102 02203-008]
The study showed that pretreatment with NOD1 agonist can significantly reduce mortality, ALT levels, and hepatocyte apoptosis induced by LPS/D-GalN, independent of TNF-alpha inhibition. NOD1 agonist pretreatment also attenuated TNF-alpha/D-GalN-induced apoptotic liver damage.
Increasing evidence suggests that NODs are involved in liver diseases; however, the underlying mechanisms remain obscure. In the present study, we analyzed the effect of NOD1 agonist pretreatment on acute liver failure induced by lipopolysaccharide (LPS) in D-galactosamine (D-GalN)-sensitized mice. We found that pretreatment with the NOD1 agonist markedly reduced LPS/D-GalN-induced mortality, elevation of serum ALT levels, and hepatocyte apoptosis. The protective effect of NOD1 agonist was independent of tumor necrosis factor (TNF)-alpha inhibition. NOD1 agonist pretreatment also attenuated TNF-alpha/D-GalN-induced apoptotic liver damage. The anti-apoptotic protein A20 expression was more pronounced in NOD1 agonist pretreated mice than in controls, and knockdown of A20 abrogated the protective effect of NOD1 agonist on LPS/D-GalN-induced liver injury and hepatocyte apoptosis. Further experiments showed that NOD1 agonist-induced A20 upregulation required the presence of kupffer cells and TNF-alpha. Taken together, our data strongly indicate that NOD1 is involved in the regulation of liver injury and could be a potential therapeutic target for liver diseases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据