期刊
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
卷 10, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13756-021-00924-9
关键词
Acinetobacter baumannii; RND-type efflux pumps; Efflux pumps inhibitor; Two-component systems
类别
资金
- Shahid Beheshti University of Medical Sciences, Tehran, Iran [11247]
The majority of Acinetobacter baumannii isolates in this study were extensively drug-resistant, with efflux pump activity observed in 40% of isolates against multiple antibiotics, mainly tigecycline. Various amino acid substitutions were detected in the products of regulatory genes, with the G186V mutation in AdeS associated with efflux pump overexpression. No insertion sequences were detected. Further studies are needed to understand the exact role of RND efflux pumps in multidrug-resistant clinical isolates of A. baumannii.
Background This study was aimed to characterize the genetic diversity and expression of three putative resistance-nodulation-cell division (RND)-type efflux systems and their contribution to multidrug efflux in clinical isolates of Acinetobacter baumannii. Methods Antimicrobial susceptibility testing of 95 A. baumannii isolates was determined by Kirby-Bauer disk diffusion for 18 antibiotics and minimum inhibitory concentration (MIC) of colistin was determined by the broth microdilution method. Moreover, the MIC of five classes of antibiotics was assessed using E-test strips in the presence and absence of phenylalanine-arginine beta-naphthylamide (PA beta N). Regulatory genes of the RND efflux pumps (adeRS, adeL, adeN and baeSR) were subjected to sequencing. The relative expression of adeB, adeG and adeJ genes was determined by quantitative real-time PCR (qRT-PCR). Results Overall, the majority of isolates (94%) were extensively drug-resistant (XDR). In the phenotypic assay, efflux pump activity was observed in 40% of the isolates against multiple antibiotics mainly tigecycline. However, we found no efflux activity against imipenem. Several amino acid substitutions were detected in the products of regulatory genes; except in AdeN. Of note, G186V mutation in AdeS was found to be associated with overexpression of its efflux pump. No insertion sequences were detected. Conclusions Our findings outlined the role of RND efflux pumps in resistance of A. baumannii to multiple antibiotics particularly tigecycline, and pointed out the importance of a variety of single mutations in the corresponding regulatory systems. Further studies are required to decipher the precise role of RND efflux pumps in multidrug-resistant clinical isolates of A. baumannii.
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