4.8 Article

Modulating the Electrical and Mechanical Microenvironment to Guide Neuronal Stem Cell Differentiation

期刊

ADVANCED SCIENCE
卷 8, 期 7, 页码 -

出版社

WILEY
DOI: 10.1002/advs.202002112

关键词

ciliary neurotrophic factor; conductive polymers; electrical stimulation; electrophysiology; graphene; cell scaffolds; stem cells

资金

  1. NIH [K08NS089976]
  2. Stanford School of Medicine Dean's postdoctoral fellowship

向作者/读者索取更多资源

The use of a conductive graphene scaffold was shown to significantly accelerate the generation of human iPSC-derived neurons, with mechanical and electrical stimuli promoting their growth and maturation through different signaling pathways.
The application of induced pluripotent stem cells (iPSCs) in disease modeling and regenerative medicine can be limited by the prolonged times required for functional human neuronal differentiation and traditional 2D culture techniques. Here, a conductive graphene scaffold (CGS) to modulate mechanical and electrical signals to promote human iPSC-derived neurons is presented. The soft CGS with cortex-like stiffness (approximate to 3 kPa) and electrical stimulation (+/- 800 mV/100 Hz for 1 h) incurs a fivefold improvement in the rate (14d) of generating iPSC-derived neurons over some traditional protocols, with an increase in mature cellular markers and electrophysiological characteristics. Consistent with other culture conditions, it is found that the pro-neurogenic effects of mechanical and electrical stimuli rely on RhoA/ROCK signaling and de novo ciliary neurotrophic factor (CNTF) production respectively. Thus, the CGS system creates a combined physical and continuously modifiable, electrical niche to efficiently and quickly generate iPSC-derived neurons.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据