期刊
ACS INFECTIOUS DISEASES
卷 7, 期 4, 页码 800-810出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsinfecdis.0c00650
关键词
Mycobacterium tuberculosis; miR-18b; HIF-1 alpha; pro-inflammatory cytokines; macrophages; signaling pathways
资金
- National Key Research and Development Program of China [2017YFD0500305]
- Natural Science Foundation of Hubei Province [2019CFB459]
- China Agricultural Research System [CARS-37]
The study revealed that miR-18b is downregulated in M.tb-infected macrophages, leading to increased expression of HIF-1 alpha and subsequent production of pro-inflammatory cytokines like IL-6, resulting in decreased bacterial survival in host cells. Additionally, the study showed activation of p38 MAPK and NF-kappa B p65 by the miR-18b inhibitor.
The modulation of the interaction between macrophages and Mycobacterium tuberculosis (M.tb) through microRNA during M.tb infection is increasingly capturing the attention of researchers. However, the potential role of microRNA-18b-5p (miR-18b) is not elucidated yet. In this study, miR-18b was found to be downregulated in M.tb-infected macrophage cell lines (THP-1 and RAW264.7) in time- and dose-dependent manners. Furthermore, when the miR-18b mimic and inhibitor and small interfering RNA hypoxia-inducible factor 1 alpha (si-HIF-1 alpha) were transfected into the macrophages separately or in combination, it was found that miR-18b targeted hypoxia-inducible factor 1 alpha (HIF-1 alpha). During M.tb infection, the decrease in the expression of miR-18b facilitated HIF-1 alpha expression, which led to the increased production of pro-inflammatory cytokines, such as IL-6, resulting in decreased bacterial survival in the host cells. Moreover, the phosphorylation of p38 MAPK and NF-kappa B p65 was activated by the miR-18b inhibitor. Our findings expand the current understanding of the M.tb-cell interaction mechanism and provide a potential target to control M.tb infection.
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