期刊
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION
卷 44, 期 9, 页码 1947-1959出版社
SPRINGER
DOI: 10.1007/s40618-021-01510-x
关键词
Growth hormone-secreting pituitary adenoma; mRNAs; Long non-coding RNAs; Differentially expressed genes; Bioinformatics
资金
- National Natural Science Foundation of China [81801369]
- Natural Science Foundation of Chongqing [cstc2019jcyj-msxmX0475]
- Nursery Project of Army Medical University [2019R054]
- Clinical Research Project of Army Military Medical University [2018XLC3049]
This study evaluated the transcriptional changes in growth hormone-secreting pituitary adenomas (GH-PAs) and identified differentially expressed genes associated with invasive GH-PAs. Functional enrichment analysis revealed suppressed pathways related to epithelial cell differentiation and development, while other pathways showed an active trend in invasive GH-PAs. The study also identified specific genes and chromosomal regions linked to the invasiveness of GH-PAs, providing new insights for understanding the underlying mechanisms of these tumors.
Purpose Growth hormone-secreting pituitary adenomas (GH-PAs) are common subtypes of functional PAs. Invasive GH-PAs play a key role in restricting poor outcomes. The transcriptional changes in GH-PAs were evaluated. Methods In this study, the transcriptome analysis of six different GH-PA samples was performed. The functional roles, co-regulatory network, and chromosome location of differentially expressed (DE) genes in invasive GH-PAs were explored. Results Bioinformatic analysis revealed 101 DE mRNAs and 70 DE long non-coding RNAs (lncRNAs) between invasive and non-invasive GH-PAs. Functional enrichment analysis showed that epithelial cell differentiation and development pathways were suppressed in invasive GH-PAs, whereas the pathways of olfactory transduction, retinol metabolism, drug metabolism-cytochrome P450, and metabolism of xenobiotics by cytochrome P450 had an active trend. In the protein-protein interaction network, 11 main communities were characterized by cell- adhesion, -motility, and -cycle; transport process; phosphorus and hormone metabolic processes. The SGK1 gene was suggested to play a role in the invasiveness of GH-PAs. Furthermore, the up-regulated genes OR51B6, OR52E4, OR52E8, OR52E6, OR52N2, MAGEA6, MAGEC1, ST8SIA6-AS1, and the down-regulated genes GAD1-AS1 and SPINT1-AS1 were identified in the competing endogenous RNA network. The RT-qPCR results further supported the aberrant expression of those genes. Finally, the enrichment of DE genes in chromosome 11p15 and 12p13 regions were detected. Conclusion Our findings provide a new perspective for studies evaluating the underlying mechanism of invasive GH-PAs.
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