4.6 Article

Clonal Interference and Mutation Bias in Small Bacterial Populations in Droplets

期刊

GENES
卷 12, 期 2, 页码 -

出版社

MDPI
DOI: 10.3390/genes12020223

关键词

experimental evolution; antibiotic resistance; millifluidics; population size; mutation bias; selection bias

资金

  1. Human Frontiers in Science Program [RGP0010/2015]
  2. Netherlands organization for scientific research within the NWO XS framework [OCENW.XS.058]

向作者/读者索取更多资源

Experimental evolution studies show that in very small populations, mutant clones with higher mutation rates can adapt faster by acquiring beneficial mutations, while wild-type populations have limited adaptive potential with inactivating mutations.
Experimental evolution studies have provided key insights into the fundamental mechanisms of evolution. One striking observation is that parallel and convergent evolution during laboratory evolution can be surprisingly common. However, these experiments are typically performed with well-mixed cultures and large effective population sizes, while pathogenic microbes typically experience strong bottlenecks during infection or drug treatment. Yet, our knowledge about adaptation in very small populations, where selection strength and mutation supplies are limited, is scant. In this study, wild-type and mutator strains of the bacterium Escherichia coli were evolved for about 100 generations towards increased resistance to the beta-lactam antibiotic cefotaxime in millifluidic droplets of 0.5 mu L and effective population size of approximately 27,000 cells. The small effective population size limited the adaptive potential of wild-type populations, where adaptation was limited to inactivating mutations, which caused the increased production of outer-membrane vesicles, leading to modest fitness increases. In contrast, mutator clones with an average of similar to 30-fold higher mutation rate adapted much faster by acquiring both inactivating mutations of an outer-membrane porin and particularly inactivating and gain-of-function mutations, causing the upregulation or activation of a common efflux pump, respectively. Our results demonstrate how in very small populations, clonal interference and mutation bias together affect the choice of adaptive trajectories by mediating the balance between high-rate and large-benefit mutations.

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