The Amazing Acrobat: Yeast's Histone H3K56 Juggles Several Important Roles While Maintaining Perfect Balance

Acetylation on lysine 56 of histone H3 of the yeast Saccharomyces cerevisiae has been implicated in many cellular processes that affect genome stability. Despite being the object of much research, the complete scope of the roles played by K56 acetylation is not fully understood even today. The acetylation is put in place at the S-phase of the cell cycle, in order to flag newly synthesized histones that are incorporated during DNA replication. The signal is removed by two redundant deacetylases, Hst3 and Hst4, at the entry to G2/M phase. Its crucial location, at the entry and exit points of the DNA into and out of the nucleosome, makes this a central modification, and dictates that if acetylation and deacetylation are not well concerted and executed in a timely fashion, severe genomic instability arises. In this review, we explore the wealth of information available on the many roles played by H3K56 acetylation and the deacetylases Hst3 and Hst4 in DNA replication and repair.

Automated summary

Acetylation of lysine 56 on histone H3 in yeast is involved in processes affecting genome stability, particularly in DNA replication and repair. This modification occurs in the S-phase of the cell cycle to mark newly synthesized histones, but is removed by deacetylases in the G2/M phase to prevent genomic instability. The roles played by H3K56 acetylation and deacetylases Hst3 and Hst4 in maintaining genome stability are crucial but not fully understood.