期刊
FRONTIERS IN PHYSIOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphys.2021.619447
关键词
DNA methylation; VEGF; change of direction; PGC1 alpha; AMPK; MAPK
类别
资金
- Malaysian government agency: Majlis Amanah Rakyat (MARA)
- United Kingdom's Engineering and Physical Sciences Research Council (EPSRC)
- United Kingdom Medical Research Council's (MRC) center for doctoral training
- North Staffordshire Medical Institute (NMSI)
- Society for Endocrinology and Glaxosmithkline
- Liverpool John Moores University (United Kingdom) The Norwegian School of Sport Sciences, Oslo (Norway)
- University of Birmingham (United Kingdom)
- Swedish Research Council for Sport Science [P2018-0097]
- Swedish Research Council (Vetenskapsradet) [2015-00165]
- Marie Sklodowska-Curie Actions (European Commission) [704978]
- Marie Curie Actions (MSCA) [704978] Funding Source: Marie Curie Actions (MSCA)
- Swedish Research Council [2015-00165] Funding Source: Swedish Research Council
The study found that different types of exercises, especially change of direction exercise, may induce higher exercise intensity and greater genome-wide hypomethylation features post-exercise, which are associated with changes in gene expression levels related to exercise adaptation.
The methylome and transcriptome signatures following exercise that are physiologically and metabolically relevant to sporting contexts such as team sports or health prescription scenarios (e.g., high intensity interval training/HIIT) has not been investigated. To explore this, we performed two different sport/exercise relevant high-intensity running protocols in five male sport team members using a repeated measures design of: (1) change of direction (COD) versus; (2) straight line (ST) running exercise with a wash-out period of at least 2 weeks between trials. Skeletal muscle biopsies collected from the vastus lateralis 30 min and 24 h post exercise, were assayed using 850K methylation arrays and a comparative analysis with recent (subject-unmatched) sprint and acute aerobic exercise meta-analysis transcriptomes was performed. Despite COD and ST exercise being matched for classically defined intensity measures (speed x distance and number of accelerations/decelerations), COD exercise elicited greater movement (GPS-Playerload), physiological (HR), metabolic (lactate) as well as central and peripheral (differential RPE) exertion measures compared with ST exercise, suggesting COD exercise evoked a higher exercise intensity. The exercise response alone across both conditions evoked extensive alterations in the methylome 30 min and 24 h post exercise, particularly in MAPK, AMPK and axon guidance pathways. COD evoked a considerably greater hypomethylated signature across the genome compared with ST exercise, particularly at 30 min post exercise, enriched in: Protein binding, MAPK, AMPK, insulin, and axon guidance pathways. Comparative methylome analysis with sprint running transcriptomes identified considerable overlap, with 49% of genes that were altered at the expression level also differentially methylated after COD exercise. After differential methylated region analysis, we observed that VEGFA and its downstream nuclear transcription factor, NR4A1 had enriched hypomethylation within their promoter regions. VEGFA and NR4A1 were also significantly upregulated in the sprint transcriptome and meta-analysis of exercise transcriptomes. We also confirmed increased gene expression of VEGFA, and considerably larger increases in the expression of canonical metabolic genes PPARGC1A (that encodes PGC1-alpha) and NR4A3 in COD vs. ST exercise. Overall, we demonstrate that increased physiological/metabolic load via COD exercise in human skeletal muscle evokes considerable epigenetic modifications that are associated with changes in expression of genes responsible for adaptation to exercise.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据