期刊
FEBS OPEN BIO
卷 11, 期 4, 页码 1054-1075出版社
WILEY
DOI: 10.1002/2211-5463.13099
关键词
in vitro replication; lesion bypass; next‐ generation sequencing; nucleotide excision repair; T antigen; translesion synthesis
资金
- National Research, Development and Innovation Fund of Hungary [2018-1.2.1-NKP-2018-00005]
- Hungarian Academy of Sciences [KEP-5/2019, PREMIUM-2017-17]
- NKFIH [K-124881, K-116072, K-123989]
- ELTE [KMOP-4.2.1/B-10-2011-0002]
- [2018-1.2.1-NKP]
This study demonstrated the applicability of an SV40 large T antigen-based replication system for studying DNA damage removal and DNA replicative bypass processes. While effective for examining NER and TLS, especially its one-polymerase branch, it is less suited for investigations of homology-related repair processes such as TSw.
DNA damage removal by nucleotide excision repair (NER) and replicative bypass via translesion synthesis (TLS) and template switch (TSw) are important in ensuring genome stability. In this study, we tested the applicability of an SV40 large T antigen-based replication system for the simultaneous examination of these damage tolerance processes. Using both Sanger and next-generation sequencing combined with lesion-specific qPCR and replication efficiency studies, we demonstrate that this system works well for studying NER and TLS, especially its one-polymerase branch, while it is less suited to investigations of homology-related repair processes, such as TSw. Cis-syn cyclobutane pyrimidine dimer photoproducts were replicated with equal efficiency to lesion-free plasmids in vitro, and the majority of TLS on this lesion could be inhibited by a peptide (PIR) specific for the pol eta-PCNA interaction interface. TLS on 6-4 pyrimidine-pyrimidone photoproduct proved to be inefficient and was slightly facilitated by PIR as well as by a recombinant ubiquitin-binding zinc finger domain of pol eta in HeLa extract, possibly by promoting polymerase exchange. Supplementation of the extract with recombinant PCNA variants indicated the dependence of TLS on PCNA ubiquitylation. In contrast to active TLS and NER, we found no evidence of successful TSw in cellular extracts. The established methods can promote in vitro investigations of replicative DNA damage bypass.
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