期刊
CARTILAGE
卷 13, 期 2_SUPPL, 页码 225S-237S出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/1947603521993217
关键词
osteoarthritis; platelet-rich plasma; mesenchymal stem cells; inflammatory environment; in vitro study
类别
OA is an inflammatory and degenerative disease with numerous treatments not fully effective. MSCs and PRP are proposed for OA treatment. APS seems to act better on inflammation front, with ADSCs responding better to the inflammatory microenvironment and APS treatment than BMSCs.
Objective Osteoarthritis (OA) is an inflammatory and degenerative disease, and the numerous treatments currently used are not fully effective. Mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) are proposed for OA treatment as biologic therapies. The aim of the study was to observe the role of autologous protein solution (APS), a type of PRP, on chondrogenic differentiation of 2 types of MSCs, from bone marrow (BMSCs) and adipose tissue (ADSCs), in an in vitro osteoarthritic microenvironment. Design Inflammatory culture conditions, mimicking OA, were obtained by adding interleukin-1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF alpha), or synovial fluid from patient osteoarthritic knees (OSF), to the culture medium. MSCs were then treated with APS. Results After 1 month of culture, both cell types formed mature micromasses, partially altered in the presence of IL-1 beta and TNF alpha but quite preserved with OSF. Inflammatory conditions hindered differentiation in terms of gene expression, not counterbalanced by APS. APS triggered type I collagen deposition and above all contributed to decrease the expression of metalloproteinases in the most aggressive conditions (IL-1 beta and TNF alpha in the culture medium). ADSCs originated micromasses more mature and less prone toward osteogenic lineage than BMSCs, thus showing to better adapt in an aggressive environment than BMSC. Conclusions APS seems to act better on inflammation front and, between cell types, ADSCs respond better to the inflammatory microenvironment of OA and to the treatment with APS than BMSCs.
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