Optimizing of Microalgae Scenedesmus sp. Biomass Production in Wet Market Wastewater Using Response Surface Methodology

The present study aimed to optimize the production of Scenedesmus sp. biomass during the phycoremediation process. The biomass productivity was optimized using face centred central composite design (FCCCD) in response surface methodology (RSM) as a function of two independent variables that included wet market wastewater concentrations (A) with a range of 10% to 75% and aeration rate (B) with a range of 0.02 to 4.0 L/min. The results revealed that the highest biomass productivity (73 mg/L/d) and maximum growth rate (1.19 day(-1)) was achieved with the 64.26% of (A) and 3.08 L/min of (B). The GC-MS composition analysis of the biomass yield extract revealed that the major compounds are hexadecane (25%), glaucine (16.2%), and phytol (8.33%). The presence of these compounds suggests that WMW has the potential to be used as a production medium for Scenedesmus sp. Biomass, which has several applications in the pharmaceutical and chemical industry.

Automated summary

This study optimized the production of Scenedesmus sp. biomass during phycoremediation by utilizing response surface methodology with face centred central composite design. The results indicated that the highest biomass productivity and maximum growth rate were achieved with specific wet market wastewater concentrations and aeration rates. GC-MS composition analysis revealed major compounds in the biomass extract, suggesting the potential use of wet market wastewater as a production medium for Scenedesmus sp. biomass with applications in pharmaceutical and chemical industries.