期刊
MICROMACHINES
卷 12, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/mi12030263
关键词
sample-in-answer-out; microfluidic; human papillomavirus (HPV); reverse dot hybridization
类别
资金
- Project of the Science and Technology Innovation of Anhui province [2017070802D146, 2018080402A009]
- Fundamental Research Funds for the Central Universities [WK911000057]
- Key Programs for Research and Development of Anhui Province [1704a0802153]
This research presents an automated microfluidic system that integrates the functions of a traditional PCR laboratory into a palm-sized microfluidic card, capable of typing 24 HPV genotypes. The system achieves a detection limit of 200 copies/test and a 100% positive detection rate for clinical samples, making it an effective method for HPV detection.
This paper proposes an automated microfluidic system for molecular diagnostics that integrates the functions of a traditional polymerase chain reaction (PCR) laboratory into a palm-sized microfluidic cartridge (CARD) made of polystyrene. The CARD integrates 4 independent microfluidic sample lanes, which can independently complete a sample test, and each sample lane integrates the 3 functional areas of the sample preparation area, PCR amplification area, and product analysis area. By using chemical cell lysis, magnetic silica bead-based DNA extraction, combined with multi-PCR-reverse dot hybridization with microarray, 24 HPV genotypes can be typing tested in CARD. With a custom-made automated CARD operating platform, the entire process can be automatically carried out, achieving sample-in-answer-out. The custom-made operation platform is developed based on a liquid handling station-type, which can automatically load off-chip reagents without placing reagents in CARD in advance. The platform can control six CARDs to work simultaneously, detect 24 samples at a time. The results show that the limit of detection of the microfluidic system is 200 copies/test, and the positive detection rate of clinical samples by this system is 100%, which is an effective method for detection of HPV.
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