期刊
MICROMACHINES
卷 12, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/mi12020161
关键词
intracellular calcium dynamics; spatio-temporal signals; ATP and shear stress stimuli; theoretical modeling; endothelial cells
类别
资金
- National Natural Science Foundation of China [11902066, 31971243]
- National Key R&D Program of the Ministry of Science and Technology of China [2020YFC2004400]
- Fundamental Research Funds for the Central Universities [DUT20RC(4)015, DUT20YG113]
This study focuses on how spatially distributed ATP and shear stress signals generated by microfluidic technology affect intracellular calcium dynamics. The results demonstrate the system's capacity to produce diverse signals, leading to different modes of calcium responses within cells.
Intracellular calcium dynamics play essential roles in the proper functioning of cellular activities. It is a well known important chemosensing and mechanosensing process regulated by the spatio-temporal microenvironment. Nevertheless, how spatio-temporal biochemical and biomechanical stimuli affect calcium dynamics is not fully understood and the underlying regulation mechanism remains missing. Herein, based on a developed microfluidic generator of biochemical and biomechanical signals, we theoretically analyzed the generation of spatio-temporal ATP and shear stress signals within the microfluidic platform and investigated the effect of spatial combination of ATP and shear stress stimuli on the intracellular calcium dynamics. The simulation results demonstrate the capacity and flexibility of the microfluidic system in generating spatio-temporal ATP and shear stress. Along the transverse direction of the microchannel, dynamic ATP signals of distinct amplitudes coupled with identical shear stress are created, which induce the spatio-temporal diversity in calcium responses. Interestingly, to the multiple combinations of stimuli, the intracellular calcium dynamics reveal two main modes: unimodal and oscillatory modes, showing significant dependence on the features of the spatio-temporal ATP and shear stress stimuli. The present study provides essential information for controlling calcium dynamics by regulating spatio-temporal biochemical and biomechanical stimuli, which shows the potential in directing cellular activities and understanding the occurrence and development of disease.
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