期刊
INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY
卷 2021, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2021/8862291
关键词
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资金
- Youth Foundation of Wannan Medical College [WK2018F05]
- Climbing Peak Training Program for Innovative Technology team of Yijishan Hospital of Wannan Medical College [KPF2019016]
Schisandrol B, a lignan isolated from dried Schisandra chinensis fruits, has been shown to have protective effects on the liver, heart, kidneys, and memory. A sensitive HPLC-MS/MS method was developed to measure Schisandrol B levels in rat plasma and tissues, allowing for assessment of its pharmacokinetics, oral bioavailability, and tissue distribution. The results showed promising pharmacokinetic profiles and tissue distribution patterns of Schisandrol B, providing valuable insights for future studies on its pharmacological and biological characteristics.
Schisandrol B, a lignan isolated from dried Schisandra chinensis fruits, has been shown to exhibit hepatoprotective, cardioprotective, renoprotective, and memory-enhancing properties. This study sought to design a sensitive and efficient HPLC-MS/MS approach to measuring Schisandrol B levels in rat plasma and tissues in order to assess the pharmacokinetics, oral bioavailability, and tissue distributions of this compound in vivo. For this analysis, bifendate was chosen as an internal standard (IS). A liquid-liquid extraction (LLE) approach was employed for the preparation of samples that were subsequently separated with an Agilent ZORBAX Eclipse XDB-C-18 (4.6 x 150 mm, 5 pm) column with an isocratic mobile phase consisting of methanol and water containing 5 mM ammonium acetate and 0.1% formic acid (90:10, v/v). A linear calibration curve was obtained over the 5-2000 ng/mL and 1-1000 ng/mL ranges for plasma samples and tissue homogenates, respectively. This established method was then successfully applied to investigate the pharmacokinetics, oral bioavailability, and tissue distributions of Schisandrol B in Sprague-Dawley (SD) rats that were intravenously administered 2 mg/kg of Schisandrol B monomer, intragastrically administered Schisandrol B monomer (10 mg/kg), or intragastrically administered 6 mL/kg SCE (equivalent to 15 mg/kg Schisandrol B monomer). The oral absolute bioavailability of Schisandrol B following intragastric Schisandrol B monomer and SCE administration was approximately 18.73% and 68.12%, respectively. Tissue distribution studies revealed that Schisandrol B was distributed throughout several tested tissues, with particular accumulation in the liver and kidneys. Our data represent a valuable foundation for future studies of the pharmacologic and biological characteristics of Schisandrol B.
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