期刊
GENETICS
卷 218, 期 1, 页码 -出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/genetics/iyab033
关键词
centromere; CENP-A; histone H4; CIN
资金
- National Institutes of Health Intramural Research Program at the National Cancer Institute
- National Institutes of Health [R01HG005853]
- Canadian Institute of Health Research [FDN-143264]
The mislocalization of overexpressed Cse4 is influenced by defects in proteolysis and sumoylation, with reduced dosage of histone H4 leading to suppression of CIN when Cse4 is overexpressed. This study identified genes that contribute to the mislocalization of Cse4 and highlighted the role of H4 in facilitating Cse4 sumoylation and mislocalization to noncentromeric regions.
Mislocalization of the centromeric histone H3 variant (Cse4 in budding yeast, CID in flies, CENP-A in humans) to noncentromeric regions contributes to chromosomal instability (CIN) in yeast, fly, and human cells. Overexpression and mislocalization of CENP-A have been observed in cancers, however, the mechanisms that facilitate the mislocalization of overexpressed CENP-A have not been fully explored. Defects in proteolysis of overexpressed Cse4 (GALCSE4) lead to its mislocalization and synthetic dosage lethality (SDL) in mutants for E3 ubiquitin ligases (Psh1, Slx5, SCFMet30, and SCFCdc4), Doa1, Hir2, and Cdc7. In contrast, defects in sumoylation of overexpressed cse4K215/216/A/R prevent its mislocalization and do not cause SDL in a psh1 Delta strain. Here, we used a genome-wide screen to identify factors that facilitate the mislocalization of overexpressed Cse4 by characterizing suppressors of the psh1D GALCSE4 SDL. Deletions of histone H4 alleles (HHF1 or HHF2), which were among the most prominent suppressors, also suppress slx5 Delta, cdc4-1, doa1 Delta, hir2 Delta, and cdc7-4 GALCSE4 SDL. Reduced dosage of H4 leads to defects in sumoylation and reduced mislocalization of overexpressed Cse4, which contributes to suppression of CIN when Cse4 is overexpressed. We determined that the hhf1-20, cse4-102, and cse4-111 mutants, which are defective in the Cse4-H4 interaction, also exhibit reduced sumoylation of Cse4 and do not display psh1 Delta GALCSE4 SDL. In summary, we have identified genes that contribute to the mislocalization of overexpressed Cse4 and defined a role for the gene dosage of H4 in facilitating Cse4 sumoylation and mislocalization to noncentromeric regions, leading to CIN when Cse4 is overexpressed.
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