期刊
FRONTIERS IN PLANT SCIENCE
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2021.613059
关键词
abscisic acid; hypoxia; metabolism; signaling; soil flooding; tomato
资金
- Spanish Ministerio de Economia y Competitividad, Universitat Jaume I
- Generalitat Valenciana/Fondo Europeo de Desarrollo Regional (FEDER) [AGL2016-76574-R, UJI-B201623, UJI-B2016-24, IDIFEDER/2018/010, UJI-B2019-24]
- UJI PICD program
- Ramon y Cajal contracts from Spanish Ministerio de Economia y Competitividad [RYC-2016-19325, RYC-201723645]
- Juan de la CiervaFormacion contract from the Spanish Ministerio de Economia y Competitividad [FJCI-2016-28601]
Soil flooding induces specific genetic and metabolic responses, with ABA playing a crucial role. ABA-deficient plants exhibit higher gas exchange parameters and metabolic alterations under waterlogging conditions, suggesting the importance of ABA in regulating these responses.
Soil flooding is a compound abiotic stress that alters soil properties and limits atmospheric gas diffusion (O-2 and CO2) to the roots. The involvement of abscisic acid (ABA) in the regulation of soil flooding-specific genetic and metabolic responses has been scarcely studied despite its key importance as regulator in other abiotic stress conditions. To attain this objective, wild type and ABA-deficient tomatoes were subjected to short-term (24 h) soil waterlogging. After this period, gas exchange parameters were reduced in the wild type but not in ABA-deficient plants that always had higher E and g(s). Transcript and metabolite alterations were more intense in waterlogged tissues, with genotype-specific variations. Waterlogging reduced the ABA levels in the roots while inducing PYR/PYL/RCAR ABA receptors and ABA-dependent transcription factor transcripts, of which induction was less pronounced in the ABA-deficient genotype. Ethylene/O-2-dependent genetic responses (ERFVIIs, plant anoxia survival responses, and genes involved in the N-degron pathway) were induced in hypoxic tissues independently of the genotype. Interestingly, genes encoding a nitrate reductase and a phytoglobin involved in NO biosynthesis and scavenging and ERFVII stability were induced in waterlogged tissues, but to a lower extent in ABA-deficient tomato. At the metabolic level, flooding-induced accumulation of Ala was enhanced in ABA-deficient lines following a differential accumulation of Glu and Asp in both hypoxic and aerated tissues, supporting their involvement as sources of oxalacetate to feed the tricarboxylic acid cycle in waterlogged tissues and constituting a potential advantage upon long periods of soil waterlogging. The promoter analysis of upregulated genes indicated that the production of oxalacetate from Asp via Asp oxidase, energy processes such as acetyl-CoA, ATP, and starch biosynthesis, and the lignification process were likely subjected to ABA regulation. Taken together, these data indicate that ABA depletion in waterlogged tissues acts as a positive signal, inducing several specific genetic and metabolic responses to soil flooding.
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