4.6 Article

Analysis of Secreted Proteins and Potential Virulence via the ICEs-Mediated Pathway of the Foodborne Pathogen Vibrio parahaemolyticus

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FRONTIERS IN MICROBIOLOGY
卷 12, 期 -, 页码 -

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FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2021.612166

关键词

Vibrio parahaemolyticus; bacterial secretion systems; secreted protein; integrative and conjugative elements; dihydrolipoamide dehydrogenase

资金

  1. National Science Foundation for Young Scientists of China [31701566]
  2. Natural Science Foundation of the Jiangsu Higher Education Institutions of China [18KJB550011]
  3. Science and Technology Planning Project of Zhoushan of China [2016C41004, 2018C21017]
  4. Research Projects of Xuzhou University of Technology [XKY2018248]

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This study identified a novel immunogenic protein DLDH associated with ICE in V. parahaemolyticus, revealing a potential relationship between ICE-associated transport and secreted proteins and enhancing the understanding of mobile genetic elements.
Vibrio parahaemolyticus uses bacterial secretion systems and integrative and conjugative elements (ICEs) to induce various diseases and to adapt to harsh environments, respectively. Information pertaining to the identity of secreted proteins and functional characterization of ICEs has been previously reported, but the relationship between these elements remains unclear. Herein we investigated secreted proteins of V. parahaemolyticus strains JHY20 and JHY20oICE using two-dimensional gel electrophoresis and LC-MS/MS, which led to the identification of an ICE-associated secreted protein - dihydrolipoamide dehydrogenase (DLDH). Considering the data related to its physical and biochemical characterization, we predicted that DLDH is a novel immunogenic protein and associated with virulence in JHY20. Our findings indicate a potential relationship between ICE-associated transport and secreted proteins and shed light on the function of such transport mechanisms. We believe that our data should enhance our understanding of mobile genetic elements.

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