期刊
FRONTIERS IN MICROBIOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2021.618352
关键词
persistence; RNA; hepatitis A; water; food contact surfaces; blueberries
类别
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC) [RDCPJ 522816-17]
- Consortium de recherche et innovations en bioprocedes industriels au Quebec [CRIBIQ 2017-038-C29]
- NSERC
- Fonds de Recherche du Quebec -Nature et Technologies
This study evaluated the persistence of heat-inactivated HAV in various conditions and found that viral RNA could be detected for up to 90 days in water and phosphate-buffered saline regardless of temperature, as well as on stainless steel and blueberries under most conditions.
Enteric viruses, such as human norovirus and hepatitis A virus (HAV), are the leading cause of transmissible foodborne illness. Fresh produce such as berries are often contaminated by infected food handlers, soiled water, or food contact surfaces. The gold-standard method for virus detection throughout the food chain is RT-qPCR, which detects portions of genomes including non-infectious viral particles and naked viral RNA. The aim of this study was to evaluate the persistence of heat-inactivated HAV in water, phosphate-buffered saline, on stainless steel and polyvinyl chloride, and on blueberries at -80 degrees C, -20 degrees C, 4 degrees C, and room temperature. In water and phosphate-buffered saline, viral RNA could be detected for up to 90 days regardless of temperature when the initial load was 2.5 x 10(4) or 2.5 x 10(6) genome copies. It was detected on polyvinyl chloride and blueberries under most conditions. On stainless steel, the large initial load persisted for 90 days, while the medium-level load was detected only up to 16 days at room temperature or 60 days at 4 degrees C. The detection of non-infectious viral RNA can confound investigations of gastroenteritis outbreaks. Pretreatments that discriminate between naked RNA, non-infectious virions and infectious virions need to be included in the RT-qPCR method in order to reduce the risk of positive results associated with non-infectious viral particles.
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