Next-Generation Sequencing Reveals the Progression of COVID-19

The novel coronavirus SARS-CoV-2 (causing the disease COVID-19) has caused a highly transmissible and ongoing pandemic worldwide. Due to its rapid development, next-generation sequencing plays vital roles in many aspects. Here, we summarize the current knowledge on the origin and human transmission of SARS-CoV-2 based on NGS analysis. The ACE2 expression levels in various human tissues and relevant cells were compared to provide insights into the mechanism of SAS-CoV-2 infection. Gut microbiota dysbiosis observed by metagenome sequencing and the immunogenetics of COVID-19 patients according to single-cell sequencing analysis were also highlighted. Overall, the application of these sequencing techniques could be meaningful for finding novel intermediate SARS-CoV-2 hosts to block interspecies transmission. This information will further benefit SARS-CoV-2 diagnostic development and new therapeutic target discovery. The extensive application of NGS will provide powerful support for our fight against future public health emergencies.

Automated summary

The ongoing COVID-19 pandemic caused by the novel coronavirus SARS-CoV-2 has highlighted the importance of next-generation sequencing in understanding the virus. NGS analysis has provided insights into the origin and transmission of SARS-CoV-2, as well as related factors like ACE2 expression levels, gut microbiota dysbiosis, and immunogenetics of patients. These sequencing techniques have the potential to identify new hosts, aid in diagnostic development, and discover therapeutic targets, offering crucial support in the fight against future public health emergencies.