期刊
TOXINS
卷 13, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/toxins13020121
关键词
Apoptosis; bovine mammary epithelial cells; endoplasmic reticulum stress; polydatin; zearalenone
资金
- Scientific Research Project of Education Department of Jilin Province [JJKH20211133KJ]
- Jilin Scientific and Technological Development Program, P.R. China [20190301034NY]
The study showed that PD can effectively alleviate ZEA-induced damage on bovine mammary epithelial cells by reducing oxidative damage and ER stress, thus reducing cell apoptosis.
Zearalenone (ZEA) is a mycotoxin of the Fusarium genus that can cause endoplasmic reticulum (ER) stress and Apoptosis in bovine mammary epithelial cells (MAC-T). Polydatin (PD), a glycoside purified from Polygonum cuspidatum, has antioxidant properties. This study aimed to explore whether PD can alleviate ZEA-induced damage on bovine mammary epithelial cells (MAC-T). We found that incasing the concentration of ZEA (0, 7.5, 15, 30, 60, 90, 120, and 240 mu M) gradually decreased the cell viability. PD treatment alone at 5, 10, and 20 mu M did not affect cell viability. Follow-up studies then applied 30 mu M of ZEA and 5 mu M of PD to treat cells; the results showed that the ZEA + PD treatment group effectively reduced cell oxidative damage compared with the ZEA treatment group. The qPCR analysis showed that ZEA treatment significantly up-regulated the expression of ER stress-related genes, relative to the control. However, adding PD significantly down-regulated the expression of ER stress-related genes. The cell apoptosis detection results showed that, compared with the ZEA treatment group, the ZEA + PD treatment group down-regulated the Bax gene and up-regulated the Bcl-2 gene expressions, which reduced the cell apoptosis rate and Caspase-3 activity. Taken together, these results indicate that PD reduces ZEA-induced apoptosis by inhibiting oxidative damage and ER stress.
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