Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya

The bioecology of phlebotomine sand flies is intimately linked to the utilization of environmental resources including plant feeding. However, plant feeding behavior of sand flies remains largely understudied for Afrotropical species. Here, using a combination of biochemical, molecular, and chemical approaches, we decipher specific plant-feeding associations in field-collected sand flies from a dry ecology endemic for leishmaniasis in Kenya. Cold-anthrone test indicative of recent plant feeding showed that fructose positivity rates were similar in both sand fly sexes and between those sampled indoors and outdoors. Analysis of derived sequences of the ribulose bisphosphate carboxylase gene large subunit (rbcL) from fructose-positive specimens implicated mainly Acacia plants in the family Fabaceae (73%) as those readily foraged on by both sexes of Phlebotomus and Sergentomyia. Chemical analysis by high performance liquid chromatography detected fructose as the most common sugar in sand flies and leaves of selected plant species in the Fabaceae family. Analysis of similarities (ANOSIM) of the headspace volatile profiles of selected Fabaceae plants identified benzyl alcohol, (Z)-linalool oxide, (E)-beta -ocimene, p-cymene, p-cresol, and m-cresol, as discriminating compounds between the plant volatiles. These results indicate selective sand fly plant feeding and suggest that the discriminating volatile organic compounds could be exploited in attractive toxic sugar- and odor- bait technologies control strategies. Author summary Plant feeding as an essential resource of sand flies, primary vectors of Leishmania parasites, is largely understudied for Afrotropical species. Here, we combined field ecology, biochemical, molecular and chemical approaches, to decipher plant feeding associations in field-collected sand flies from a dry ecology endemic for leishmaniasis in Kenya revealing i) similar rates of plant feeding among sand fly sexes sampled from indoor and outdoor environments, ii) Acacia plants in the family Fabaceae as those readily foraged on by sand fly species in Phlebotomus and Sergentomyia, iii) fructose as the common sugar in sand flies and leaves of selected plant species in the Fabaceae family, iv) compounds namely benzyl alcohol, (Z)-linalool oxide, (E)-beta -ocimene, p-cymene, p-cresol, and m-cresol, as discriminating volatile organic compounds between volatiles of selected Fabaceae plants. The findings indicate selective sand fly plant feeding and suggest that the discriminating volatile organic compounds could be exploited in attractive toxic sugar- and odor-bait technologies for sand fly control.

Automated summary

The study reveals plant feeding associations in field-collected sand flies from a dry ecology endemic for leishmaniasis in Kenya, showing similar rates of plant feeding among sand fly sexes sampled from indoor and outdoor environments, mainly foraging on Acacia plants in the family Fabaceae. The results suggest that discriminating volatile organic compounds from selected Fabaceae plants could be used in sand fly control strategies.