期刊
ENVIRONMENTAL MONITORING AND ASSESSMENT
卷 188, 期 2, 页码 -出版社
SPRINGER
DOI: 10.1007/s10661-016-5099-4
关键词
Industrial waste effluent; Long-term pollution; Soil enzyme activity; 16S rRNA gene; Denaturing gradient gel electrophoresis; Biological indicators
资金
- Chinese Academy of Sciences (CAS), Beijing, China
- Academy of Sciences for the Developing World (TWAS), Trieste, Italy
- National Natural Science Foundation of China [41322007]
- Foundation for Innovative Research Groups of the National Natural Science Foundation of China [51221892]
Although numerous studies have addressed the influence of exogenous pollutants on microorganisms, the effect of long-term industrial waste effluent (IWE) pollution on the activity and diversity of soil bacteria was still unclear. Three soil samples characterized as uncontaminated (R1), moderately contaminated (R2), and highly contaminated (R3) receiving mixed organic and heavy metal pollutants for more than 20 years through IWE were collected along the Mahi River basin, Gujarat, western India. Basal soil respiration and in situ enzyme activities indicated an apparent deleterious effect of IWE on microbial activity and soil function. Community composition profiling of soil bacteria using 16S rRNA gene amplification and denaturing gradient gel electrophoresis (DGGE) method indicated an apparent bacterial community shift in the IWE-affected soils. Cloning and sequencing of DGGE bands revealed that the dominated bacterial phyla in polluted soil were affiliated with Firmicutes, Acidobacteria, and Actinobacteria, indicating that these bacterial phyla may have a high tolerance to pollutants. We suggested that specific bacterial phyla along with soil enzyme activities could be used as relevant biological indicators for long-term pollution assessment on soil quality.
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