On-site genetic analysis for species identification using lab-on-a-chip

This paper presents a microfluidic device capable of performing genetic analysis on dung samples to identify White Rhinoceros (Ceratotherium simum). The development of a microfluidic device, which can be used in the field, offers a portable and cost-effective solution for DNA analysis and species identification to aid conservation efforts. Optimization of the DNA extraction processes produced equivalent yields compared to conventional kit-based methods within just 5 minutes. The use of a color-changing loop-mediated isothermal amplification reaction for simultaneous detection of the cytochrome B sequence of C. simum enabled positive results to be obtained within as little as 30 minutes. Field testing was performed at Knowsley Safari to demonstrate real-world applicability of the microfluidic device for testing of biological samples.

Automated summary

This study developed a microfluidic device for genetic analysis of White Rhinoceros dung samples, providing a portable and cost-effective solution for species identification to aid conservation efforts. Optimization of DNA extraction processes and the use of specific amplification reactions allowed for accurate detection results to be obtained quickly.