期刊
ECOLOGY AND EVOLUTION
卷 11, 期 4, 页码 1535-1543出版社
WILEY
DOI: 10.1002/ece3.7053
关键词
conservation genetics; identification; lab-on-a-chip; microfluidics; rhinoceros
This study developed a microfluidic device for genetic analysis of White Rhinoceros dung samples, providing a portable and cost-effective solution for species identification to aid conservation efforts. Optimization of DNA extraction processes and the use of specific amplification reactions allowed for accurate detection results to be obtained quickly.
This paper presents a microfluidic device capable of performing genetic analysis on dung samples to identify White Rhinoceros (Ceratotherium simum). The development of a microfluidic device, which can be used in the field, offers a portable and cost-effective solution for DNA analysis and species identification to aid conservation efforts. Optimization of the DNA extraction processes produced equivalent yields compared to conventional kit-based methods within just 5 minutes. The use of a color-changing loop-mediated isothermal amplification reaction for simultaneous detection of the cytochrome B sequence of C. simum enabled positive results to be obtained within as little as 30 minutes. Field testing was performed at Knowsley Safari to demonstrate real-world applicability of the microfluidic device for testing of biological samples.
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